Expression, purification, and detection of novel streptococcal superantigens.
نویسندگان
چکیده
منابع مشابه
Functional characterization of streptococcal pyrogenic exotoxin J, a novel superantigen.
Streptococcal toxic shock syndrome (STSS) is a highly lethal, acute-onset illness that is a subset of invasive streptococcal disease. The majority of clinical STSS cases have been associated with the pyrogenic toxin superantigens (PTSAgs) streptococcal pyrogenic exotoxin A or C (SPE A or C), although cases have been reported that are not associated with either of these exotoxins. Recent genome ...
متن کاملIdentification & characterisation of the two novel streptococcal pyrogenic exotoxins SPE-L & SPE-M.
BACKGROUND & OBJECTIVES The streptococcal pyrogenic exotoxins (SPEs) are produced by Streptococcus pyogenes and belong to the family of bacterial superantigens, a group of highly mitogenic proteins. The aim of this study was to search unfinished streptococcal genomes for novel superantigens, to generate recombinant proteins from potential open reading frames (ORFs) and to analyse them for super...
متن کاملSeparation of T-cell-stimulating activity from streptococcal M protein.
The superantigenic properties of M protein type 5 of Streptococcus pyogenes have been implicated as an important pathogenicity factor in streptococcal autoimmune diseases. Here we show that after a single purification step by affinity chromatography on immobilized albumin or fibrinogen, M protein has no mitogenic activity for T cells. We demonstrate that the superantigenicity of M proteins of t...
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Background & Aim: Bacterial superantigens, stimulate polyclonal T cells irrespective of their antigen specificity, resulting in a massive release of cytokines from T cells and monocytes, and suggest that that they could be candidates of new antitumor agents. Recent attempts have been done to specifically target superantigens towards tumors. Here, we evaluate TGFαL3-SEB fusion protein as a new a...
متن کاملPyrogenicity and cytokine-inducing properties of Streptococcus pyogenes superantigens: comparative study of streptococcal mitogenic exotoxin Z and pyrogenic exotoxin A.
Streptococcal mitogenic exotoxin Z (SMEZ), a superantigen derived from Streptococcus pyogenes, provoked expansion of human lymphocytes expressing the Vbeta 2, 4, 7 and 8 motifs of T-cell receptor. SMEZ was pyrogenic in rabbits and stimulated the expression of the T-cell activation markers CD69 and cutaneous lymphocyte-associated antigen. A variety of cytokines was released by human mononuclear ...
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عنوان ژورنال:
- Methods in molecular biology
دوره 214 شماره
صفحات -
تاریخ انتشار 2003